Effects of cultivation medium and conditions on the production of alpha-galactosidase by Aspergillus awamore & A/ carbonarius

Abstract

The α-gal production by Aspergillus awamori and A. carbonarius (local isolates) as affected by several culture conditions was studied showing that 0.5 % corn bran and 2.0 % cane baggase were used for the production of α-gal by Aspergillus awamori & Aspergillus carbonarius respectively; the best pH value, incubation temperature and incubation period for the production of α-gal were 4.8, 30º C and 6 days for both experimental fungi; raffinose at the concentration 1 % supplemented with 0.5 % corn bran and acacia gum at concentration 1 % supplemented with 2 % cane baggase were the best synthetic carbon source for the production of α-gal for Aspergillus awamori and Aspergillus carbonarius respectively; L-Asparagine (0.262 %) was the best concentration of nitrogen source for Aspergillus awamori whereas sodium nitrate (0.4 %) was the best concentration of nitrogen source for Aspergillus carbonarius and the best ratio of surface area to volume of medium was 0.525 for Aspergillus carbonarius and 2.260 for Aspergillus carbonarius.

Egyptian Journal of Biotechnology Vol. 25 (1) 2007: pp. 60-69