Biodegradation of agro-industrial orange waste under solid state fermentation and natural environmental conditions

Abstract

Evaluation of the possibility of the re-use of agro-industrial orange peel and pulp wastes under solid state fermentation and natural environmental condition as a source of enzymes production [α & β amylase, cellulase, pectinase(s), lipase(s), esterase(s) and peroxidase(s)] the physiological enzymes of lysis and total protein. Different microorganisms such as fungi, bacteria and yeast which were charged of waste analyse and have ability to produce previous enzymes and protein. These microorganisms were isolated from the fermented waste and preliminarily identified to test each one of them for their enzymes production and also to test them for inducing the fermentation process under the natural conditions, or by using one of the present enzyme producer strain. The protein and physiological enzymes production were electro-phoretically patterned in different bands. And finally, the possibility to use the fermented waste as a bio-fertilizer was done and it stimulated the growth of wheat plant using water culture, especially after the toxicity of the fermented waste was investigated.


KEY WORDS: Solid state, peel & pulp waste, enzymes, protein, bio-fertilizer, bacteria, fungi, yeast.


Egyptian Journal of Biology Vol.4 2002: 23-30