M Sudhakar
Shri Vishnu College of Pharmacy, Vishnupur, Bhimavaram 534 202 Andhra Pradesh, India
CV Rao
National Botanical Research Institute, Ranapratap Marg, Lucknow 226 001
AL Rao
Shri Vishnu College of Pharmacy, Vishnupur, Bhimavaram 534 202 Andhra Pradesh, India
A Ramesh
Shri Vishnu College of Pharmacy, Vishnupur, Bhimavaram 534 202 Andhra Pradesh, India
N Srinivas
Department of Physiology, University of Tüebingen, Tbingen, Germany
DB Raju
Shri Vishnu College of Pharmacy, Vishnupur, Bhimavaram 534 202 Andhra Pradesh, India
B Krishna Murthy
Department of Physiology, University of Tüebingen, Tbingen, Germany
Abstract
The effect of Callistemon lanceolatus (Syn. C. citrinus curtis; Family: Myrtaceae) leaf oil was studied for the antinociceptive and anti-inflammatory activity in experimental animals. C. lanceolatus, 25 – 100 mg/kg administered orally for 3 days exhibited graded dose response equivalent to 21.95% - 89.90% protection in the tail flick latent test in rat. The C. lanceolatus oil (50 and 100 mg/kg, given orally for 3 days) was effective in hot plate reaction time (64.05% and 112.97%, p<0.01 and p<0.001), analgesymeter induced mechanical pain (28.17% and 54.42%, p<0.01 and p<0.001) and acetic acid- induced writhing (26.68% and 51.79%, p<0.5 and p<0.05) in mice. The oil of C. lanceolatus potentiated the analgesic activity with pentazocine (10 mg/kg, i.p.) and aspirin (25 mg/kg, i.p.). In the carrageenan-induced paw edema C. lanceolatus oil (50 and 100 mg/kg, given orally for 3 days) decreased paw volume significantly (26.68% and 51.79%) and dose dependent anti-inflammatory activity in 1-3 hour time interval and potentiated with nimesulide (50 mg/kg, p.o.). In summary, this study demonstrates that leaf oil of C. lanceolatus has significant antinociceptive and anti-inflammatory activity.
Keywords: Callistemon lanceolatus, oil, pain, inflammation
East and Central African Journal of Pharmaceutical Sciences Vol. 7 2004: 10-15