A High-performance Thin Layer Chromatography Densitometric Method for the assay of Mebendazole Tablets

Abstract

A simple, precise and accurate high-performance thin layer chromatographic (HPTLC) method was developed for the assay of mebendazole tablets. The separation was carried out by using HPTLC Silica gel 60 F254, (20 × 10 cm) with 250 μm thickness using ethyl acetate - ammonia 25% solution (25:0.5) as a mobile phase. HPTLC separation of the drug was followed by densitometry measurement at 310 nm. Mebendazole was satisfactorily resolved with retention factor (Rf) values of 0.51 ± 0.02. The method complied with International Conference on Harmonization acceptance
criteria for linearity (250 - 600 ng), precision, accuracy and specificity.

Key words: HPTLC, method validation, assay, mebendazole