Method for Determination of Tinidazole using Direct UV-Visible Spectrophotometry and Differential Spectrophotometry in Pure and Tablet Dosage Forms

Abstract

Three simple, rapid, selective, precise and accurate spectrophotometric methods for the determination of tinidazole in tablet formulation were developed. The first method was based on the direct absorbance measurements of tinidazole in 0.5 N NaOH with a λmax of 368.6 nm and linearity range of 20 – 150 μg/ml. The second method was based on direct measurement of absorbance at 279.2 nm for tinidazole in 0.5 N HCl whereby the linearity range was 50-150 μg/ml. The third method was based on the differential spectra between tinidazole solution in 0.5 N NaOH and 0.5 N HCl. The maxima recorded was 368.8 nm while the minima was 276 nm with a linearity range of 20 – 120 μg/ml. The methods were validated by determining accuracy, precision, limit of detection, limit of quantitation and performing recovery studies. The developed methods were successfully applied in the analysis of commercial samples of tinidazole and could therefore be used in the routine analysis of tinidazole formulations.

Keywords: Tinidazole, direct colorimetry, UV-visible spectrophotometry, differential spectrophotometry

East and Central African Journal of Pharmaceutical Sciences Vol. 14 (2011) 75-80