Evaluation of the Markers of Oxidative Stress and Hepatocellular Injury in Diabetic Wistar rats fed on Black Finger Millet (Eleusine coracana) Seed Coat Matter.

Abstract

Excessive generation of reactive oxygen species (ROS) can cause diseases by damaging cellular integrity. Antioxidant-rich foods may help  prevent the development of diabetes mellitus (DM) by counteracting these harmful effects. The study aimed to assess oxidative stress  markers and hepatocellular injury in diabetes mellitus rats on a Finger millet seed coat (FMSC) diet. Precisely, 20% and 40% of FMSCM  were formulated by blending 400 g and 300 g of rat chow with 100 g and 200 g of SCM, respectively. Exactly 10 mL and 15 mL of clean  water were introduced into the mixture to make a paste. Five groups of five male rats were used in this study. The 2nd-5th groups were injected with 60 mg/kg b.w of streptozotocin to trigger diabetes. The 1st group was fed with 100% rat chow, while the 2nd group was not  treated. The 3rd-4th groups were fed with 20% and 40% of FMSC, respectively, and the 5th group received standard medication. The rats were fed for six weeks and then sacrificed, and the blood sample was collected. Biochemical analyses were performed using standard procedures. Results from the study revealed that ingestion of seed coat matter (SCM) markedly reduced the levels of the aforementioned  enzymes. Observations showed that while MDA levels were substantially lowered in groups fed with SCM, CAT and SOD  activities were markedly raised. This study has ascertained that black finger millet SC can counteract oxidative stress and its attendant   consequences.