Isolation and Molecular Detection of Multidrug Resistant Klebsiella Pneumoniae Harboring BlaSHV Gene, from Suspected Respiratory Disease Patients Attending Some Hospital in Dutse Metropolis, Jigawa State

Abstract

Klebsiella pneumoniae is a Gram-negative opportunistic bacterium that causes infections in hospitalized and immune compromised  individuals. Currently, K. pneumoniae is showing a high resistance to a broad spectrum of drugs including beta-lactam antibiotics,  Penicilline, fluoroquinolones, and aminoglycosides. This resistance is resulting in a growing worldwide problem regarding the choice of  effective antibiotic treatment for hospital-acquired infections. Meanwhile, the current study was carried out for the isolation and  molecular detection of multidrug resistant Klebsiella pneumoniae harboring blaSHV gene, in Federal University Dutse Teaching Hospital  and Dutse General Hospital respectively, in Jigawa State, Nigeria. In this research a total of 100 sputum samples were collected and analyzed using standard microbiological methods. Antibiotic susceptibility test was carried out using modified Kirby-Baur disk diffusion  method, the blaSHV gene was detected using PCR. The results showed 16% confirmed K. pneumoniae. Dutse General Hospital were  recorded with 9(56.25%) while Federal University Dutse Teaching Hospital 7(43.75) isolates. A 50-59 years and above 60 years age groups  had the highest occurrence of 4(25%) . Males had higher occurrence of 11(68.75) compared to female gender (31.25). The K. pneumoniae  were found to be more resistant to Ampiclox 62.5%, Cefuroxime and nalidaxic acid 56.26% each, it was highly susceptible to imipenem  100%, levofloxacin 100%, cefotaxime 93.75, nitrofurantoin 75% and gentamycin 75%.