Larvicidal activity, phytochemical and GC-MS analysis of crude extract derived from Ceriop decandra (Griff) against Aedes aegypti (Linnaeus)

Abstract

Mosquito borne diseases are on increase, couple with increasing effects of chemical control agents which is leading to environmental pollution. This necessitated the use of natural and eco-friendly sources for mosquito control. Record from local inhabitants have shown that certain shrubs and herbs can effectively control mosquito infestations. This present research screened the leaf, bark and root extract of Ceriops decandra (Griff) a mangrove specie from the state of Terengganu in Malaysia against third instar larvae of Aedes aegypti. Screening was carried out in accordance with World Health Organization (WHO) protocol of GC-MS analysis having 39 displayed metabolites from the leaf with 2-pyrrolidinone, 1-methyl as the major constituent, 25 metabolites from the bark with 4-O -methyl mannose as its major constituent. The root has 23 metabolites with 1-propene, 3-[(4nitrobutyl) thio as the major constituent, the probit analysis of the three plant parts showed that the leaf has the highest value with mortality of 83% to larvae with lethal concentration value of LC50 34.27±0..90 ppm, the root has 76% mortality and LC50 of 45.13±1.00 ppm and bark has the least value with 60% mortality rate of larvae, LC50 78.20±1.02 ppm. The result showed that the plant could play a major role as a sustainable and alternative control in place of chemical insecticides.