Liquid Chromatography Mass Spectrometer (Lc/Ms) profile revealed flavonoids and terpenoids with antioxidant potential in aqueous fraction of Combretum micranthum leaf extract

Abstract

Combretum micranthum (CM) is commonly used for its ethno-medicinal potentials without much or no scientific basis. Thus, the aim of this research is to evaluate aqueous fraction of C. micranthum leaf extract for possible antioxidant compounds using liquid chromatography mass spectrometer technique. In vitro antioxidant was carried out using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Ferric Reducing Power (FRAP) assay methods and the results indicated the free radical scavenging activity of 80.351±0.732 and 0.800± 0.00 at 2.50mg/m1 concentrations which is significantly different when compared to reference standard of 84.718±0.605 and 0.426±0.000 (ascorbic acid) respectively, with concentration or dose-dependent. LC-MS profile revealed the presence of medicinally important compounds like Myricetin (Flavone), Epioxylubimin (Sesquiterpenoids), Furocoumaric acid (Phenolic glycoside) among others. The findings of this study revealed that C. micranthum is rich in potential metabolites and was reflected as its antioxidant potential and could be used for drug and other oxidative stress related management.