Extraction and Quantification of Bisphenol-A Level in Infant Polycarbonate Feeding Bottles using High Performance Liquid Chromatography Technique

Abstract

Polycarbonate plastics (PC) containing bisphenol A (BPA) are used for the production of infant feeding bottles, food storage containers, kitchen utensils and some components of medical devices. Trace amount of BPA have been reported in water and food products kept in PC containers. The leaching of bisphenol A from new and used polycarbonate feeding bottles into 10% ethanol (EU regulated simulant) and hot water was investigated using HPLC analysis. The bottles were filled to the corresponding nominal volumes with 10% ethanol, allowed to stand for 24hours and with boiled HPLC grade water for 2hours at 40°C, followed by extraction with ethyl acetate. The results indicated the release of high amount of BPA from new feeding bottles than the previously used ones. The BPA leachate in 10% ethanol was within the range of 20-61ng/mL and 14.13-27.30ng/mL in new and used bottle respectively, while the range in hot water was 12.53-92.65 ng/mL and 7.81-55.02ng/mL in new and used bottles respectively. The validation parameters were Limit Of Detection: 1.14 ng/mL, Limit Of Quantification: 3.80 ng/mL, while the percent recovery for spiked samples and efficiency of the extraction procedure were 98 –103 % and %RSD ranged between 2.00 – 5.61%.The estimated tolerable daily intake(TDI) using the average BPA leachate in boiled water has exceeded the new revised TDI of 4μg/kgbw/day established by European Food Safety Authority (EFSA).