Phytochemical and antimicrobial activity of securidaca longipedunculata root against urinary tract infection pathogens

Abstract

Urinary tract infection caused by bacteria leads to inflammation and over growth of uropathogens and prevalence of infection for both genders, but women is more vulnerable especially at the sexually active ages. But unfortunately, the continuous emergence of antibiotic resistant bacterial strains brings most serious public health concerns. It is therefore, important to look for more alternative, effective, safer and safer treatments. The aim of the present study was to investigate antimicrobial activity of Securidaca longipedunculata root extracts against some human pathogenic bacteria and fungi using agar well diffusion method and agar dilution for minimum inhibitory concentration (MIC). Phytochemical and acute toxicity studies were carried out using the standard methods. Phytochemicals which include alkaloids, flavonoids, saponins, tannins, carbohydrates and triterpenes were detected in both aqueous and methanolic extracts. The antimicrobial results revealed that, the methanolic extract had promising antibacterial activity. For intense Escherichia coli was found to be the most susceptible bacteria in both methanol and aqueous extracts at 500mg/ml with inhibition zones of 20 mm and 16mm, Staphylococcus aureus was next most susceptible bacteria to methanol extract of the root with inhibition zone of 16 mm and MIC of 31.25 mg/ml respectively. The extracts does not showed activity against all the tested fungal isolates at lowest concentration 62.5 mg/ml. The LD₅₀ of Securidaca longipedunculata was found to be greater than 5000 mg /kg and could be considered safe for consumption.