Antioxidant and Antimicrobial Activities of Green Synthesized Silver Nanoparticle Using Moringa Oleifera Seeds Extracts

Abstract

Nanotechnology deals with materials in nanoscale that exhibit incredible chemical, physical and biological properties. This study is aimed at synthesizing silver nanoparticles from Moringa oleifera seed using aqueous and ethanol as solvents and compare their antioxidant as well as antimicrobial activities of these two synthesized nanoparticles. The synthesized nanoparticles were subjected to preliminary characterization using UV- spectroscopy to ensure their formation which were confirmed by attaining the plasmon resonance surface of both particles at 320nm. Standard assay for antioxidant scavenging of ferric ion and DDPH were employed and standard methods for drop plate technique, minimum inhibition concentration and minimum bactericidal/fungicidal concentrations were used. The results indicate that silver nanoparticles synthesized from ethanol extract (EEMS) has excellent ferric ion scavenging activity compared to silver nanoparticles synthesized from aqueous extract (AEMS), while AEMS has 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging potential compared to EEMS. Both nanoparticles show the antimicrobial efficiency were dose dependent but more inhibition zone was observed on EEMS using drop plate technique at 25mg/dl against all the organisms used (S. aureus, E. coli and C. albican). And in minimum inhibition concentrations, the result shows both particles inhibit the growth of all organisms at 6.25 µg/dl except against E. coli which is at 12.5 µg/ml by EEMS. Similarly, minimum bactericidal / fungicidal concentrations were the same in all organisms at 12.5 µg/ml except against C. albican which was at 6.25 µg/ml by EEMS. The finding revealed that both EEMS and AEMS are good antioxidants and antimicrobial agents and their activities are concentration dependent.