Isolation and characterization of some flavonoids from the leaf of Tapinanthus globiferus growing on Acacia nilotica

Abstract

Tapinanthus globiferus is used ethnomedicinally for the treatment of bacterial infections, inflammation, stomach pain, ulcers among others. The aim of the study was to isolate bioactive compounds from the leaf of T. globiferus growing on Acacia nilotica. The powdered plant material was extracted with 90 % methanol using cold maceration and the resulting crude methanol leaf extract was partitioned into n-hexane, chloroform, ethylacetate and n-butanol fractions. The ethylacetate fraction was chromatographed on a silica gel, sephadex LH-20 column and preparative thin-layer chromatography. (-)-Epicatechin and Quercetin 3-O-β-D-glucopyranoside were isolated and characterized by means of physiochemical and spectroscopic (1D and 2D-NMR) analyses for the first time from T. globiferus growing on A. nilotica.

Keywords:  Tapinanthus globiferus; Flavonoids; Isolation; NMR