Laccase Facilitated Synthesis of Silver Nanoparticles for Biomedical, Biocontrol andDyeDegradation Applications

Abstract

Green synthesis of various metallic nanoparticles has gained significant attention in recent yearsdue to their numerous applications. Laccase synthesis of silver-nanoparticles (AgNPs), characterization and applications were investigated. The synthesized AgNPs were characterizedusing UV-visible spectroscopy, FTIR, SEM and EDS. Antimicrobial activity of the synthesizedAgNPs against Escherichia. coli, Staphylococcus aureus, Colletotrichum sp., Fusariumsp., Penicillum and Aspergillus niger was studied. Disease suppression capability of AgNPs onchili pepper and pear fruits were monitored by visual observation. A yellow to brown colour changeand surface plasmon  resonance peak at 407 nm indicated AgNPs formation. The FTIRspectraofthe AgNPs reveals the appearance of distinctive bands, SEM  analysis showed a particle size(20nm-100 nm) with spherical morphology while EDS reveals a strong signal in the silver regionand  confirm the formation of AgNPs. The AgNPs showed growth inhibition of bacteria(33-42%), fungi (12-33%) and dyes degradation  (25-63%). Furthermore, effective disease suppression was observed on pear and chili pepper fruits. This study described the  synthesisofAgNPs using laccase from A. carbonarious and emphasizes its role in disease suppressionandindustrial dye degradation