Acute toxicity, preliminary phytochemistry and anti-peptic ulcer study of the bi-herbal mixture of Persea americana (Lauraceae) seed and Citrus sinesis (Rutaceae) peel using swiss albino mice

Abstract

Background: The first known type of medicine is the usage of herbs and has been practiced in every culture. In various systems of traditional medicine as well as in current "western" medicine, plants and the constituents of their secondary metabolites have a long history of use and are the source of significant medications.
Aim: This study was aimed to investigate the acute toxicity, phytochemical composition and anti-peptic ulcer effect of the Bi-herbal mixture of the methanol extract of avocado (Persea americana) pear seed and orange (Citrus sinesis) peel on peptic ulcer induced in albino Swiss mice.
Methods: Phytochemical constituents of the bi-herbal formulation, evaluation of the acute toxicity effect of the bi-herbal formulation and anti-peptic ulcer studies using Hydrochloric acid mixed with ethanol and Indomethacin induced peptic ulcers in albino Swiss mice were carried out using standard procedures.
Results: Results obtained from the phytochemical study showed that Cardiac Glycoside, Flavonoids, Eugenols and Alkaloids where highly present, Saponins, Phenolics and Terpenoids where present, while Tannins was absent. The acute toxicity study revealed that the LD50 was more than 2000 mg/kg as none of the animals died. While the anti-ulcer study revealed dose dependent gastro-protective activities of the extract in both indomethacin and Ethanol/HCl induced peptic ulcers.
Conclusion: It is concluded that the methanol extract of the Bi-herbal formulation of pear seed and orange peel, possess anti-ulcer activity and clinical trials can be extended on peptic ulcer patients.