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Jul 18, 2024Keywords:
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Effects of ethanolic shoots extract of Borassus aethiopum on some haemostatic parameters and liver histology in Wistar rat
Abstract
Background: Borassus aethiopum shoot is called muruchi in Hausa, used as Aphrodiasic, antibiotic as food locally consumed and cultivated in many parts of Africa. Haemostasis is an important mechanism for the control of bleeding through the use of vWF, F V, F VII or F VIII (Kalot et al., 2022). Liver is an important organ for detoxification (Mujahid et al., 2017).
Aim: The study aimed at determine the effects of ethanolic shoots extract of B. aethiopum on Haemostatic Parameters and Liver histology in wistar rats.
Methodology: For the acute toxicity study, the median lethal dose (LD50) was determined using Lorke's method while for Sub acute toxicity study, twenty (20) males Wistar rats weighed 120-200g were divided into four groups of five rats (n=5) each. The groups 1 (control), 2, 3 and 4 were received 2 ml Distilled water, 300, 600 and 1,200 mg/kg of extract for 28 days. Body weight recorded daily. The haemostatic parameters were analyzed using ELISA (Enzyme linked immunosorbent assay) method. Liver histology were examined using method of Microscopy.
Results: The LD50 was determined at greater than (˃5,000) mg/kg. No mortality was recorded after toxicity Study. No significant change in the body weight. There was no significant increase in haemostatic parameters; von Willebrand factor (vWF), Clotting Factor V (Labile factor) (FV), Clotting Factor VII (Tissue factor) (FVII) and Clotting Factor VIII (Antihaemophilic factor) in the treated groups compared to control group (p>0.05). The histology of Liver microscopy showed histopathological changes with periportal inflammations, unremarkable hepatocytes compared to the control group.
Conclusion: This finding shows that the ethanolic shoots extract of B. aethiopum did not exert significant changes in the haemostatic parameters compared to the control group. However, histopathological changes were observed in the liver of Wistar rats.
