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Aug 22, 2023Keywords:
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Testicular histology and oxidative stress in models of sleep deprivation: The role of zinc supplementation
Abstract
Background: The current global population works and lives under a 24/7 lifestyle which is characterized by increased physical activities, reduced rest and sleep. About 30 percent of employed adults are Sleep deprived, either by deliberately extending working hours into the night as in Insufficient Sleepers (IS) or works during the night-time as in Night Shift Workers (NSW). Coincidentally, there is remarkable decline in male fertility rates in these modern societies, attributed to testicular failure which is believed to be due to lifestyle modifications.
Aim: The Aims of the study were to create IS and NSW models of SD to study their effects on Testicular morphology and Testicular tissue oxidative stress biomarkers, and to study the role of concomitant Zinc supplementation on those effects.
Methods: Forty (40) adult male Wister rats were randomly divided into five groups: Control, NSW, NSWZ, IS and ISZ models. NSW and NSWZ models were subjected to 12 hours SD, while IS and ISZ models were subjected to 18 hours SD using Modified Multiple Platform (MMPM) method daily for 56 days. ISZ and NSWZ models were supplemented with Zinc sulphates (5mg/animal/day). Testicular tissue MDA, Total Antioxidant Capacity (TAC) and Testicular histology were evaluated and compared.
Results: SD in both NSW and IS models resulted in significant increase (p<0.05) in testicular tissue MDA compared to control. SD in both NSW and IS models, resulted in significant decrease (p<0.05) in testicular tissue TAC compared to control. Plate I (Control): The histology Photomicrograph shows normal testicular histoarchitecture. Plate II (NSW) and Plate IV (IS): shows seminiferous tubules (ST) with relatively scanty spermatids and Sertoli cells with area of cellular degeneration in Plate IV (IS).
Conclusion: The IS model of SD is more detrimental to testicular integrity than the NSW model, with Zinc supplementation ameliorating some of these effects.
