Antibacterial properties of Lawsonia Inermis against biofilm producing methicillin-resistant Staphylococcus aureus In Ahmadu Bello University Teaching Hospital Zaria, Nigeria

Abstract

Background: The treatment options for MRSA are getting narrower by the day, which is associated with MRSA turning multi-drug resistance organisms,  causing increased mortality around the globe. Biofilm formation by MRSA worsens the situation by rendering it impenetrable, making the treatments  more complex.

Aim: To evaluate the antibacterial properties of Lawsonia inermis against Biofilm producing MRSA among burns/wound patients and health-care workers  at Ahmadu Bello University Teaching Hospital Zaria.

Materials and Methods: The study was conducted on 300 participant, 94 Health Care Workers and 206 Burn Wound Patient. S. aureus was cultured on  Mannitol salt agar while MRSA was detected using Cefoxitin disc. The plant was extracted using Soxhlet technique and antibacterial activity of the plant  was tested using Kirby-Bauer technique by testing 3 different concentrations of the extract (2400, 2800, and 3200) µg. the biofilm formation studies was  done using TCP technique.

Results: From the 94-samples collected from HCW 32 and 17 S. aureus and MRSA was detected respectively while from the 206 samples collected from  BWP 36 and 26 S. aureus and MRSA was detected respectively, among the MRSA isolated all were Biofilm producers. Increase in zone of inhibition was  observed when the isolates were tested against increasing concentration of L. inermis (2400µg 7±4mm, 2800µg 10±3mm, 3200µg 12±4mm).

Conclusion: The extracts of L. inermis have shown antibacterial activity against Biofilm producing MRSA.