Histomorphological study of lead-induced lungs lesion of mice treated with Moringa oleifera leaves extracts

Abstract

Background: Moringa oleifera leaves extracts are currently among the most common medicinal plants used to cure some diseases. Many studies have been conducted on the benefits of Moringa oleifera leaves extracts but only few studies focused on its Antifibrotic effects on lung tissue.

Aim: To study the Histomorphological changes associated with Moringa oleifera Leave extracts on lead-induced lung lesion of Mice.

Materials and Method: Twenty adult mice were divided into four groups, three test groups (A, B and C) and a control group (D). The test groups were administered orally with 5mg/kg lead acetate and 10mg/kg Moringa oleifera leaves extract concurrently; 5mg/kg lead acetate only; 10mg/kg Moringa oleifera leaves extract only; while the control group did not receive any intervention. The intervention period lasted for three weeks. On the twenty-two day of the research, the animals were sacrificed; lung tissues were harvested, processed for histological examination and stained using Hematoxylin and Eosin staining technique.

Result: Group A animal administered with lead acetate and Moringa oleifera leaves extract concurrently, shows that the deleterious effects of Lead were ameliorated, with an improve histological features of the lungs when compared with that of the Group B. while an Ill defined alveoli, thinned alveoli wall, sacs emphysema and distortion of the parenchyma cells that constitute the lung tissue are seen in test Group B, administered with lead acetate only. A remarkable, intact lung alveoli, lung parenchyma cells were observed to in test Group C and very much correlated to that of the control group.

Conclusion: Moringa oleifera administration reduced the severity of lead’s damaging effects to the lungs, and may likely exhibits anti-fibrotic as well as prophylactic effects by repairing damaged alveoli and in the latter case by preserving and /or regenerating the lung tubules.

Key words: Anti-fibrotic; lead-induced; lung; lesion; Moringa oleifera