Dyslipidaemic, oxidative stress and immunoinflammatory alterations in a rat model of late-night eating

Abstract

Background: Modern-humans have adapted a 24/7 active and feeding lifestyles with consequential eating at odds with the circadian system that threatens to pose a pandemic of metabolic diseases. Since nocturnally restricted feeding promotes growth and metabolic fitness and that adlibitum feeding disturbs diurnal rhythms and metabolic health in rodents, the use of adlibitum controls in metabolic researches can have doubtful extrapolative conclusions. Here, we simulated human late-night eating using feed restricted controls with the primary aim of exploring possible dyslipidaemic, oxidative stress and immunoinflammatory alterations of Late-night eating in Wistar rats.

Methods: Sixteen (16) male Wistar rats (aged 8-10 weeks) were randomly assigned into control or late-night eating group (n=8). Fasting weight and blood glucose were obtained and Lipids were analyzed using their respective Randox kits. Malondialdehyde, catalase and superoxide dismutase activities were assayed while Full blood counts and CD 4+ Tcells were determined using automated analyzers. Data were analyzed using SPSS V20.0, compared using Student’s t-test and significance set at p≤ 0.05.

Results: Our findings have demonstrated that late-night eating is associated with an overall significant decrease in total feeds intake, Fasting blood glucose, High density lipoprotein, catalase, and CD4+ cell counts. On the other hand, Cardiac risk ratio and Atherogenic coefficient are marginally raised, while Platelet lymphocyte ratio, Monocyte lymphocyte ratio and Monocyte High density lipoprotein ratio are insignificantly higher among the late-night eating rats than in controls.

Conclusion: Although our finding could not demonstrate an elevated risk of obesity or diabetes, it has uncovered the dyslipidaemic, oxidative stress and immunoinflammatory alterations associated with late-night eating.

Keywords: CD4 lymphocyte count; Complete blood count; Late-night eating; Lipid ratios; Oxidative stress.