Effect of cimetidine on some hematological indices of Wistar rats: modulatory role of vitamin c

Abstract

Introduction: Cimetidine is a drug used in the treatment of dyspepsia, a clinical condition with widespread distribution. Many studies have reported cimetidine to cause many unwanted effects. However, in most of those studies cimetidine was administered at higher than therapeutic doses.

Aim: The aim of this study was, therefore, to evaluate the effect of chronic cimetidine treatment at therapeutic dose on some hematological indices and the possible modulatory role of vitamin C on any such effect.

Experimental design and Animal grouping: Forty adult male Wistar rats were divided into four groups (n = 10) and treated orally for 60 days with distilled water (control); cimetidine (30 mg kg-1); cimetidine (30 mg kg-1) + vitamin C (25 mg kg-1) and cimetidine (30 mg kg-1) + vitamin C (50 mg kg-1). At the end of the study blood was collected for analysis.

Results: Total white blood cell (WBC) count (5.99 ± 0.20 x 103/mm3) of the cimetidine only-treated group was significantly lower than that of the control (7.95 ± 0.29 x 103/mm3).However, the valuesof red blood cell (RBC) count, packed cell volume (PCV), hemoglobin (Hb) concentration, mean corpuscular volume (MCV), mean corpuscular hemoglobin(MCH), mean corpuscular hemoglobin concentration (MCHC), and color index of the experimental groups were not significantly different from that of the control. Treatment with vitamin C modulated the cimetidine-induced decrease in total WBC count.

Conclusion: It was concluded that chronic cimetidine administration at therapeutic dose caused a significant decrease in WBC count and this was modulated by vitamin C.

Key words: Cimetidine; WBC count; RBC count; hemoglobin and vitamin C.