Impaired haematological parameters in chloramphenicol induced pancytopenic rabbits supplemented with Solanecio Biafrae (wòròwó) leaf extract

Abstract

Background: Solanecio biafrae is a common vegetable cooked as sauce to support pounded yam, semovita in addition to traditional, but no scientific claim in the treatment of pancytopenia (including Anemia).While chloramphenicol is a potent pancytopenia inducing drugs.
Aim: This work was designed to determine effect of ethanolic and aqueous extract of solanecia biafrae (wòròwó leaf extracts) in the treatment rabbits induced with pancytopenia using chloramphenicol overdose.
Materials and methods: Fifteen rabbits grouped into A( 5 rabbits fed with normal meal and water for 14 days), B1 ( 5 rabbits administered with 2000mg/kgBW of chloramphenicol orally for 14 days), B2 ( 5 rabbits treated with 400mg/kgBW of Wòròwó ethanolic extract daily for 14 days after 14days of post chloramphenicol inducement). C1 (5 rabbits given 2000mg/kgBW of chloramphenicol orally for 14 days), C2 (5 rabbits treated with 400mg/kgBW of aqueous extract of Wòròwó daily for 14 days after 14 days of post chloramphenicol inducement. Evaluation of Euglobulin lysis time, Haptoglobin and PCV were carried out using MIDRAY auto hematology analyzer.
Results: The result obtained showed a significant increase in Euglobulin lysis time and Haptoglobin level in rabbits overdosed with 2000mg/kg BW of chloramphenicol for five
days ( B1 and C1) and decrease in PCV compared with rabbits fed with normal meal and water for five days (A) p < 0.05.there was also a significant increase in PCV and decrease in Euglobulin lysis time and Haptoglobin in rabbits overdosed with 2000mg/kgBW chloramphenicol and treated with 400mg/kgBW of aqueous and ethanolic extract of Wòròwó ( B2 and C2) compared to when rabbits were overdosed with 2000mg/kgBW of chloramphenicol for fourteen days( C1 and B1) p< 0.05.
Conclusion: The study shows that the Supplementation with 400mg/kgBW of Solanecio biafrae given for another 14days has increased the PCV, decreased the Euglobulin lysis
time and Haptoglobin.