Uncoupling proteins 2 and 3 alter mitochondrial membrane phospholipid fatty acyl composition in yeast

Abstract

Mouse UCP1, human UCP2 and UCP3 were expressed in yeast (Saccharomyces cerevisiae). Total phospholipids were extracted and their fatty acyl composition was analysed. Expression of hUCP2 and hUCP3 significantly altered the mitochondrial phospholipid fatty acyl composition. Palmitoleoyl groups (16:1(n-7)) decreased while oleoyl groups (18:1(n-9)) increased, leading to a significant increase in the 18:1(n-9)/16:1(n-7) ratio and in the average chain length. Expression of mUCP1 did not cause any significant changes in fatty acyl composition

Keywords: yeast, Saccharomyces cerevisiae, phospholipid, fatty acyl group, uncoupling protein, adenine nucleotide translocase, desaturase

Biokemistri Vol. 17(2) 2005: 83-94