Toxicological effects of polyherbal formulations for malaria, yellow fever and haemorrhoids in Ilorin metropolis, Nigeria, on male Wistar rats: A comparative biochemical and histological study

Abstract

This study evaluated the toxicological effects of polyherbal formulations used for the treatment of malaria (Agbo iba, AI), yellow fever (Agbo kojupon, AK) and haemorrhoids (Agbo jedi, AJ) on the function indices and histoarchitecture of liver and kidney of male Wistar rats. Forty rats were randomised into two groups (A and B) of twenty animals each such that the 20 rats in group A were further reassigned into A1, A2, A3 and A4 and administered distilled water (0.5 ml), and AI, AK and AJ at 21 mg/kg body weight respectively for 4 days. Animals in group B were correspondingly assigned and treated like those of group A except that the administration lasted for 10 days. The results revealed that AI, AK and AJ contained alkaloids, saponins, phenolics and flavonoids whilst anthraquinones were not detected. The AI and AK contained tannins whereas AK and AJ contained cardiac glycosides. The AI, AK and AJ reduced the liver- and kidney-body weight ratios, activities of both the liver alkaline phosphatase and aspartate aminotransferase, levels of serum total protein, albumin, globulin, total bilirubin and conjugated bilirubin, and increased the activities of liver alanine aminotransferase, serum alkaline phosphatase, serum aspartate aminotransferase, Na+ and Cl- on days 4 and 10. The AK and AJ significantly (p<0.05) reduced the activity of serum alanine aminotransferase and increased K+. The AK, AI and AJ increased serum creatinine content on day 4 whereas only AK increased it by day 10. Serum uric acid was not altered by all the treatment on days 4 and 10. The administration of AK and AJ induced moderately swollen hepatocytes, congested and dilated blood vessels in the liver as well as imposed glomeruli and renal tubules in the kidney whereas the lobules, glomeruli and tubules were within normal liver and kidney histology after the administration of AI. Overall, the AK and AJ exhibited both functional and structural toxicities whilst the AI displayed only functional toxicity at 21 mg/kg body weight and during the 10 days of administration. The Agbo jedi exhibited the highest degree of toxicity whilst the Agbo iba was the least toxic with respect to the dose and duration of the study.