Effects of ethanolic and aqueous leaf extracts of Bryophyllum pinnatum on haematological parameters of normal and streptozotocin–induced diabetic rats

Abstract

The effect of the oral administration of both ethanolic and aqueous leaf extracts from Bryophyllum pinnatum (BP) on haematological parameters of normal and streptozotocin - induced (STZ) diabetic rat was investigated. Twenty-five male Wister rats were used and divided into five groups of five rats each. They were designated as (Normal Control – NC, Diabetic Control – DC, Diabetic Ethanolic Extract – DEE, Diabetic Aqueous Extract – DAE, and Normal Aqueous Extract – NAE). Groups NC and DC served as ‘control’ animals receiving food and water only. Groups DC, DEE and DAE were injected intraperitoneally with 65mg/kg body weight streptozotocin. Induction of diabetes mellitus was confirmed after 48 hours using glucose test strips. The test rats were all treated with 100mg/kgbwt ethanolic and aqueous leaf extracts of Bryophyllum pinnatum for 28days. At the end of the 28days, the rats were sacrificed and whole blood collected for Haematological assay. Results obtained showed a significant difference(P<0.05) in White Blood Cell (WBC), Red Blood Cell (RBC), Platelet (PLT), Haemoglobin (HBG), Packed Cell Volume (PCV), Mean Cell Volume (MCV), Mean Cell Haemoglobin (MCH), Mean Cell Haemoglobin Concentration (MCHC) and Lymphocytes (LYM) in treated rats with BP leaf extracts when compared to the diabetic and normal control groups.