Effect of acid and alkaline hydrolysis on the concentrations of albumin and globulin in Thevetia peruviana seed cake protein extract

Abstract

Thevetia peruviana seeds cake were defatted and then treated with varying concentrations each of hydrochloric acid, sodium hydroxide and calcium hydroxide solutions. Each product of hydrolysis was extracted with chloroform to isolate aglycones, the toxins of the seed. Various concentrations of hydrochloric acid and sodium hydroxide solution effected complete detoxification. Only 0.4M and 0.5M of calcium hydroxide solution detoxified the seeds completely. Albumin and globulin determination by biuret method confirmed that various concentrations of the hydrolyzing agents increased the quantity of extractable albumin and globulin in the cake. Each solution used for the detoxification had closely related trend on the total albumin and globulin value of the treated cake. Higher quantities of albumin and globulin were recorded in the samples treated with various concentrations of calcium hydroxide solutions. The study suggests that calcium hydroxide at high concentrations appear to be the best detoxicant.

Keywords: Thevetia peruviana; detoxification, albumin, globulin