Molecular characterisation of Paradiplozoon barbi (Reichenbach-Kline, 1951) from River Perak Reservoirs, Malaysia

Abstract

The paper presents the first molecular analysis of Paradiplozoon barbi from the gills of Cyprinid fishes in Bersia, Chenderoh and Temengor Reservoirs, Perak. The ITS2 rDNA gene of P. barbi was amplified using PCR to obtain the ITS2 rDNA sequence. The 778bp (ITS2 rDNA) sequence were phylogenetically compared with related species in GenBank database by maximum likelihood method(ML) and pairwise comparisons using Kimura-2 parameter model. The ITS2 rDNA of P. barbi showed no identical sequence with closely related species. The new sequence was deposited in GenBank with accession number MN688771.