Bulletin of the Chemical Society of Ethiopia
Journal / Bulletin of the Chemical Society of Ethiopia / Vol. 24 No. 1 (2010) / Articles
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DOI:
10.4314/bcse.v24i1.52967
Keywords:
High performance liquid chromatography <i>Scutellaria baicalensis georgi</i> Baicalin

Article Details

Issue
Vol. 24 No. 1 (2010)
Section
Articles

Main Article Content

Evaluation of baicalin in Scutellaria baicalensis Georgi using HPLC method


Liao Weiping
Topsun Science and Technology Stock Co., Ltd xi'an Pharmaceutical Plant, Xi’an city, Shsnxin Province, P. R. China 710075
Rui Yukui
College of Resources and Environmental Sciences, China Agricultural University, Beijing, P. R. China 100193

Abstract

High-performance liquid chromatography (HPLC) has been evaluated for the determination of components in Scutellaria baicalensis georgi, primarily by the separation of baicalin. The samples were extracted at reflux by methanol, and the content of chlorogenic acid was analyzed by HPLC. A Diamonsil C18 column (4.6 mm x 150 mm, 5 μm) was used as the analytical column. The mobile phase was methanol, water and phosphoric acid (47:53:0.2). The detection wavelength was 280 nm to determine the content of baicalin. The baicalin showed linearity over the range of 0.12-1.2 μg. Its average recovery was 98.6% and RSD was 0.78%.

KEY WORDS: High performance liquid chromatography, Scutellaria baicalensis georgi, Baicalin

Bull. Chem. Soc. Ethiop. 2010, 24(1), 115-119.

Journal Identifiers


eISSN: 1726-801X
print ISSN: 1011-3924
 
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