Antibacterial and antioxidant activity of plant-mediated green synthesized silver nanoparticles using Cissus quadrangularis aqueous extract

Abstract

Plant-based green-synthesized silver nanoparticles (AgNPs) are valuable because of their advantages over traditional methods. This study examines the environment-friendly synthesized AgNPs using Cissus quadrangularis (CQ) stem aqueous extract. The optimum parameters for AgNP synthesis were a volume of plant extract (15 mL), aq. AgNO₃ concentration (0.10 mM), AgNO₃ volume (20 mL), reaction time (10 min), pH (11), and temperature (45 ^oC). The synthesized AgNPs at optimum conditions were characterized through UV-Visible spectroscopy, FTIR, XRD, and SEM. In colloidal solutions, the occurrence of CQ-AgNP was studied through the color change measured by the UV-visible spectrum, which confirms the developed AgNPs with a typical resonance spectrum (at 430 nm). The percentage yield of synthesized AgNPs was also estimated as 94.32%. The FTIR spectra exhibit the availability of CQ phytochemicals responsible for the reduction, capping, and stabilization of Ag⁺ of CQ-AgNP. The XRD pattern and SEM results show that the generation of highly crystalline spherical shapes of AgNPs has a mean diameter of 8.68 nm. Also, CQ-AgNPs had significant antibacterial activity against S. aureus and E. coli. The CQ-AgNPs had better antioxidant activity compared to the ascorbic acid standard. Thus, this study recommends that the presently studied CQ-AgNPs may be used as a remedial agent for biomedical applications.

KEY WORDS: Antibacterial, Antioxidant, Cissus quadrangularis, Green synthesis, Silver nanoparticles.

Bull. Chem. Soc. Ethiop. 2025, 39(3), 483-502.                                                             

DOI: https://dx.doi.org/10.4314/bcse.v39i3.8