A study on the development of scaffold fabrication using citric acid polyester – nanohydroxyapatite composite

Abstract

ABSTRACT. Nano hydroxyapatite (n-HAp) and its composites have shown a great development in the field of tissue regeneration and in controlled drug delivery due to its good biocompatibility and bioactivity behavior. Furthermore, HAp-based nanocomposites enhance mechanical properties. These synthetic HAp nanocomposites can also be tailored to fabricate scaffold with controlled porosity which facilitate the growth of the cell in the field of tissue engineering. In this paper, we focus on the synthesis of nano hydroxyapatite (n-HAp) by sol-gel method. The synthesized nano powders were calcined at 500 ^oC and characterized by FT-IR, XRD and TEM. We have also described the synthesis of citric acid-based polyester by melt polycondensation method without adding catalyst. The monomers used were citric acid, 1,6-hexane diol and sebacic acid. The corresponding synthesized n-HAp/polyester composite have potential application in soft tissue engineering. The structures of polyester and its nanocomposite were studied by FT-IR and ¹H NMR spectral studies. The thermal and mechanical properties of polyester, composites and cytotoxicity activity (MTT assay) using vero cells were also studied. Porous scaffold of the nano HAp/Polyester was fabricated by solvent-casting particulate leaching technique which is useful in the development of tissue engineering applications. SEM and TEM studies were carried out for nano HAp, polyester, composites and scaffold.

KEY WORDS: Cytotoxicity, Polycondensation, Sol-gel, Solvent-casting, Tissue engineering

Bull. Chem. Soc. Ethiop. 2022, 36(4), 923-934.                                                               

DOI: https://dx.doi.org/10.4314/bcse.v36i4.17