Solid-phase extraction of folic acid from pharmaceutical formulations using modified magnetic iron oxide nanoparticles

Abstract

ABSTRACT. A new analytical approach was suggested for the extraction and determination of folic acid in pharmaceutical samples using solid-phase microextraction. Magnetic iron oxide nanoparticles modified with sodium dodecylbenzene sulfonate (Fe₃O₄@SDBS) were used for the adsorption of folic acid (FA) from an aqueous solution and determined spectrophotometrically at λ_max of 365 nm. The chemical and physical conditions that may affect the efficiency of extraction were studied and optimized, such as the pH of the solution, surfactant and adsorbent amount, extraction time, and desorption factors. A good linearity range of 0.2-6 μg/mL with correlation coefficient higher than 0.999 and limit of detection of 0.08 μg/mL were obtained, in addition to high extraction efficiency of 98% and enrichment factor 15. The method exhibited good accuracy and precision with recoveries ranged 98-102% and intraday precisions of best than 3.5% at all concentrations. The method was successfully applied for the determination of folic acid in pharmaceutical samples within a limited separation time.

KEY WORDS: Folic acid, MNPs, Extraction, SDBS, Spectrophotometry, Solid phase extraction

Bull. Chem. Soc. Ethiop. 2022, 36(2), 291-302.                                                                

DOI: https://dx.doi.org/10.4314/bcse.v36i2.4