Preparation and assessment of prostaglandin E2 formulated with hydroxypropyl methyl cellulose and span 60 sustained release pessaries (vaginal suppositories)

Abstract

Prostaglandin E2 (PGE2) is presented as a composed release pessaries utilizing two-polymer base. Hydroxypropyl methyl cellulose HPMC was used as a viscosity modifier and bioadhesive agent. Furthermore, nonionic surfactant span 60 (Sp 60) with or without cholesterol (CH) mixed with the base was used. The defined pessaries were assessed with regards to hardness, weight variation, softening time test and in vitro release studies. Every one of the formulations demonstrated consistency with pharmacopoeial benchmarks. In vitro expressed PGE2 disintegration was completed in phosphate buffer pH 4.5 utilizing USP XXII basket apparatus. TEM demonstrated that the PEG/Sp 60 bases formed niosomal vesicles upon hydration with water while the WH15/Sp 60 gave rise to emulsions when melted in phosphate buffer pH 4.5. Both types of the novel composed release pessaries (WH15/Sp 60 and WH15/HPMC) indicated moderate release rates for PGE2 when no CH detected and the recipes WH15/30%HPMC and PEGIII/SP60 being used as an effective matrix previously to give PGE2 sorting out the release. The outcomes also demonstrated that the CH addition to either WH15 or PEG base brought about increased PGE2 release rates. No interaction amongst PGE2 and excipients were observed from DSC studies.

KEY WORDS: Pessary, PGE2, WH15, HMPC, Sp 60, Polymer, Sustained release, TEM

Bull. Chem. Soc. Ethiop. 2018, 32(2), 361-370.

DOI: https://dx.doi.org/10.4314/bcse.v32i2.14