Ideal hepatotoxicity model in rats using Carbon Tetrachloride (CCl4)

Abstract

A study to produce ideal Hepatotoxicity rats’ models using varying concentrations of carbon tetrachloride (CCl₄) was carried out. A total of seventy five rats were divided into five (5) groups of twenty five (25) rats each; rats in group I are negative control, were not induced with lipid peroxidation. Rats in groups II, III, IV and V were induced with lipid peroxidation and liver damage using, 90, 105 and 120mg/Kg body weight CCl₄ respectively. Five (5) rats were removed from each group after 48 hours, 72 hours (3 days) and 144 hours (6 days) of inducement with CCl₄ and sacrificed for blood sample collection. Sera obtained were analyzed for alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) activities, and for Malondialdehyde (MDA), total protein (TP) and albumin (ALB) concentrations. After 48hours, rats treated with various concentrations of CCl₄ had mean serum activities/ levels of AST, ALT, ALP, MDA, TP and ALB significantly higher (p< 0.05) than normal rats. The control group had 39.5±0.71, 11.5±2.12, 36±7.07U/L, 0.0769 ± 0.015 μM, 43.5±3.54, 19 .00 ± 2.83 g/L respectively. Rats treated with 75mg of CCl₄ showed activities/level non significant (p>0.05) increase of serum AST, ALT, ALP, and MDA after 96 hours of CCl₄ treatment compared with control group. However, rats treated with 90, 105 and 120mg/kg of CCl4 showed persistently high serum AST, ALT, ALP in U/L, MDA in μM/L, TP and ALB in g/L. even at 144 hours after the treatment. Ideal hepatotoxicity rat model using CCl₄ requires a dosage that can cause massive liver damage.

Key Words: carbon tetrachloride, hepatotoxicity rats modeling, natural healing.