Potency of partially purified anthocyanin from leaf extract of Guiera senegalensis against carbon tetrachloride-induced lipoperoxidation in rats

Abstract

Anthocyanin was extracted and partially purified from the ethanolic leaf extract of Guiera senegalensis (GS). The recovered anthocyanin extract was found to have a concentration of 8mg/cm3 and an absorption spectrum with peak at 268nm. In order to assess the preventive action of the anthocyanin against lipoperoxidation, serum malondialdehyde (MDA) was analyzed in rats administered with 1mg/kg of the anthocyanin before induction of lipoperoxidation in the rats using carbon tetrachloride (CC14). Serum MDA was also analyzed in rats administered with 1 and 2 mg/kg of anthocyanin for up to seven (7) days after induction of lipoperoxidation in the rats in order to determine the curative effect of the extract. The results suggest that anthocyanin extracted from GS leaves are more effective in the cure than prevention of lipoperoxidation, and the most effective daily dose of treatment is 1mg/kg for 7 days.

Key words: Guiera senegalensis, Anthocyanin, Potency, Lipoperoxidation