Antibacterial properties and preliminary phytochemical analysis of methanolic extract of mistletoe (Tapinanthus bangwensis

Abstract

Tapinanthus bangwensis (Mistletoe) is a parasitic plant grown on citrus plant and a variety of other plants, used by most communities in Nigeria for the treatment and management of ailments such as diabetics, blood pressure, asthma, epilepsy, cancer of the ovary and breast and acquired immunodeficiency syndrome (AIDS). The study was aimed at investigating the antibacterial properties and the phytochemical constituents of the leaves extract. Crude extract of Tapinanthus bangwensis leaves were screened for its antibacterial and phytochemical properties on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Proteus mirabilis and Klebsiella pneumonia. The results showed that the extract at varying concentrations has antibacterial activity on the test organisms. The extract inhibited the growth of the bacterial isolates in a concentration dependent manner with MICs (minimum inhibitory concentration) ranging from 10 – 50mg/ml. Partial purification (Thin Layer Chromatography) of the crude extract revealed five (5) components. Characteristics of these components were viewed by Infrared and UV/VIS spectrophotometer showing the presence of alkaloid, saponin, tannin, steroid and flavonoid. The observed antibacterial activity was believed to be due to the presence of the phytochemical constituents.

Keywords: Herbalism, Loranthanceae, Phytochemicals, Test organisms