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Prevalence ESBLs Isolates Augmentin Ceftazidime Ceftriaxone
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Prevalence of extended spectrum β-lactamases (esbls) among enterobacteriaceae in murtala mohammed specialist hospital, Kano, Nigeria
Abstract
Confirmed variants of enterobacteriaceae isolated from 143 patients that attended Murtala Mohammed Specialist Hospital Kano, were screened for extended spectrum β-lactamases (ESBLs) production using Clinical Laboratory Standards Institute (CLSI) breakpoint. Suspected ESBLs
producers were subjected to confirmation using Disc Replacement Method (DRM). Standard discs of augmentin {AMC 30μg (Oxoid, England)}, ceftriaxone {AUF 30μg (Oxoid, England)} and ceftazidime {RP 30μg (Oxoid, England)} were used in the screening. Of the 143 isolates screened, 114 (79.72%) were Gram negative isolates belonging to the family enterobacteriaceae. Among the
enterobacteriaceae isolates screened, the results of CLSI breakpoint test showed that 76 (66.7%) were ESBLs producers viz: Citrobacter spp. (3), Enterobacter spp. (2), E. coli (28), Klebsiella spp. (18), Morganella morganii (7), Proteus spp. (13), Salmonella spp. (1), Serratia spp. (1), Shigella spp. (2) and Yersinia spp (1). On subjecting the CLSI positive isolates to DRM, only 47 (41.2%) were confirmed ESBLs producers. These include; Citrobacter spp. (1), E. coli (20), Klebsiella spp. (12), Morganella morganii (4), Proteus spp. (8), Salmonella spp. (1) and Shigella spp. (1). ESBLs occur at an alarming rate among enterobacteriaceae isolates in Kano which calls for government intervention in the healthcare setting.
producers were subjected to confirmation using Disc Replacement Method (DRM). Standard discs of augmentin {AMC 30μg (Oxoid, England)}, ceftriaxone {AUF 30μg (Oxoid, England)} and ceftazidime {RP 30μg (Oxoid, England)} were used in the screening. Of the 143 isolates screened, 114 (79.72%) were Gram negative isolates belonging to the family enterobacteriaceae. Among the
enterobacteriaceae isolates screened, the results of CLSI breakpoint test showed that 76 (66.7%) were ESBLs producers viz: Citrobacter spp. (3), Enterobacter spp. (2), E. coli (28), Klebsiella spp. (18), Morganella morganii (7), Proteus spp. (13), Salmonella spp. (1), Serratia spp. (1), Shigella spp. (2) and Yersinia spp (1). On subjecting the CLSI positive isolates to DRM, only 47 (41.2%) were confirmed ESBLs producers. These include; Citrobacter spp. (1), E. coli (20), Klebsiella spp. (12), Morganella morganii (4), Proteus spp. (8), Salmonella spp. (1) and Shigella spp. (1). ESBLs occur at an alarming rate among enterobacteriaceae isolates in Kano which calls for government intervention in the healthcare setting.
