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Affinity chromatography photo-periodism isolation phenol-detergent Stresssteadiness Uracil-sepharose wheat
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The effect of light, temperature and desiccation on differential stability of mrna, from spring and winter wheat seedlings
Abstract
The research focused on working out a molecular method of assessing stress-steadiness and photoperiodism
of wheat and Barley varieties Selekcia KN11 grown under stress conditions. A total RNA preparation was isolated from spring and winter seedlings using phenol-detergent method, and the isolated poly (A) RNA were recovered from the total RNA using Affinity chromatography with poly
Uracil sepharose as an absorbent. One of the objectives of the study is to adapt mini preparation procedure for the effective and rapid isolation of poly (A) RNA, from total RNA preparation. Variable yields of poly (A) RNA were obtained from four days old seedlings of winter and spring
wheat and barley varieties, Celekcia KN11 which were sown under various environmental condition, the poly (A) RNA was studied and analyzed and the findings for photo-periodism of wheat varieties: krasnodarskaya-39, Bezostaya-1 and Drujna showed that the former two varieties are highly light sensitive (long day) while the latter is moderately long day variety. Variety Spectra
was found to be neutral to light (photo-periodism). On the other hand, the result of 3 varieties krasnodarskaya-39, Olimpiya and Bezostaya-1is consistent with the findings of conventional breeding for the assessment of wheat stress-steadines. The three varieties are all relatively
resistant to drought in the following order: Krasnodarskaya-39 > Olimpiya > Bezostaya-1 at P<0.05.
of wheat and Barley varieties Selekcia KN11 grown under stress conditions. A total RNA preparation was isolated from spring and winter seedlings using phenol-detergent method, and the isolated poly (A) RNA were recovered from the total RNA using Affinity chromatography with poly
Uracil sepharose as an absorbent. One of the objectives of the study is to adapt mini preparation procedure for the effective and rapid isolation of poly (A) RNA, from total RNA preparation. Variable yields of poly (A) RNA were obtained from four days old seedlings of winter and spring
wheat and barley varieties, Celekcia KN11 which were sown under various environmental condition, the poly (A) RNA was studied and analyzed and the findings for photo-periodism of wheat varieties: krasnodarskaya-39, Bezostaya-1 and Drujna showed that the former two varieties are highly light sensitive (long day) while the latter is moderately long day variety. Variety Spectra
was found to be neutral to light (photo-periodism). On the other hand, the result of 3 varieties krasnodarskaya-39, Olimpiya and Bezostaya-1is consistent with the findings of conventional breeding for the assessment of wheat stress-steadines. The three varieties are all relatively
resistant to drought in the following order: Krasnodarskaya-39 > Olimpiya > Bezostaya-1 at P<0.05.
