Spectrophotometric determination of proteins associated with virulence in Nigerian strains of aeromonas species

Abstract

Twenty six Aeromonas isolates from fishes, poultry and humans in Zaria were quantified for total soluble proteins (enzymes) profiles in January, 2007 by spectrophotometric (Biuret) method. Isolates were grown on Brain Heart Infusion (BHI) broth, they were incubated at 370C and centrifuged at 1,000 g/dl using harous labofuge. The results indicated in poultry, virulent proteins were: A. hydrophila (3.58 g/dl) A. caviae (4.00 g/dl), A. salmonicida, (3.82 g/dl) and A. sobria (0.00 g/dl). In fish, the virulent proteins were: A. hydrophila (3.11 g/dl), A. sobria (4.63 g/dl), A. caviae (2.95 g/dl) and A. salmonicida (2.74 g). In humans, the virulent proteins were: A. hydrophila (4.07 g/dl), A. sobria (3.58 g/dl) and A. caviae (3.99 g/dl). These strains of Aeromonas species were known to produce pathogenic factors which could be involved in aeromoniasis.

Key words: Quantification, Soluble proteins, Aeromonas