Effects of ethyl acetate leaf extracts of Vitex simplicifolia on antioxidant vitamins, sod, liver glycogen content and lipid profile in alloxan induced - diabetic wistar rats

Abstract

The effects of oral administration of ethyl acetate leaf extract of Vitex simplicifolia on vitamins A, E and C, Superoxide dismutase (SOD) and lipid profile levels in alloxan induced diabetic Wistar rats were investigated. The study was conducted with 30 Wistar rats, assigned into six groups of five rats each, and daily administration of ethyl acetate leaf extract of Vitex simplicifolia for 21 days was done. Diabetes mellitus was induced in overnight fasted rats by a single intraperitoneal injection (i.p) of 150 mg/kg body weight of alloxan monohydrate. Group 1 was the normal control group 2 was the diabetic control, group 3 was administered 10mg/kg of glibenclimide (positive control) and groups 4, 5, and 6 were administered 250,500 and 1000mg/kg body weight of aqueous leaf extracts of Vitex simplicifolia respectively. There were no significant changes (p<0.01) in the levels of total cholesterol (TC), triglycerol (TAG), low density lipoprotein – cholesterol (LDL-C), high density lipoprotein –cholesterol (HDL –C), SOD, vitamins A, E and C for the animals administered with the extract compared with the control groups. The result of this study suggests that aqueous leaf extract of Vitex simplicifolia has no antioxidant and hypolipidemic effect.

Keywords: Vitex simplicifolia, SOD, Lipid Profile, Antioxidant Vitamins