Effect of aqueous extract of Brysocarpus coccineus (Connaraceae) on oxidative stress biomarkers in isoniazid induced oxidative stress in adult male wistar rats

Abstract

The study was aimed at evaluating the effect of aqueous extract of Brysocarpus coccineus (Connaracea) on oxidative stress biomarkers in isoniazid induced oxidative stress in adult male Wistar rats. Adult male Wistar rats (n = 36), weighing 80g-150g were divided into six groups of six rats each and treated as follows: Group I received 1ml/kg normal saline; group II: received 27mg/kg isoniazid (INH); group III: received 27mg/kg isoniazid (INH) + 20mg/kg Livolin forte; group IV: received 27mg/kg isoniazid (INH) + 200mg/kg B. coccineus; group V: received 27mg/kg isoniazid (INH) + 400mg/kg B. coccineus; group VI: received 27mg/kg isoniazid (INH) + 800mg/kg B. coccineus. Administration was done orally twice a day for a period of 30days; isoniazid was administered at 0900hrs while livolin forte and B. coccineus were administered at 1800hrs daily. At the end of the experiment, the male Wistar rats were sacrificed and the blood was collected for assay of oxidative stress biomarkers. The results obtained showed a statistically significant (p ≤ 0.05) increase in serum Malondialdehyde in the Isoniazid group and an insignificant (p ≤ 0.05) decrease in serum Malondialdehyde levels in the groups treated with aqueous extracts of B. coccineus, the extracts also caused an insignificant (p ≤ 0.05) increase in both the serum Superoxide dismutase at 200mg/kg and serum Catalase at 400mg/kg when compared to the control and Livolin forte. The studies showed that aqueous extract of B. coccineus has anti-oxidant effects in isoniazid induced oxidative stress in adult male Wistar rats.

Keywords: Isoniazid, Oxidative stress, Brysocarpus coccineus, anti-oxidants, Livolin forte