Evaluation of antiulcer activity of aqueous ethanol extract of Thesium viride on ethanol and aspirin induced models in rats

Abstract

Thesiumviride Hill (Santalaceae) is a sub-shrub hemiparasite that grows up to 45cm tall and widely distributed in Europe, Asia and Africa. It is used in treatment of ulcer and jaundice. Phytochemical screening was carried out on the aqueous ethanol extract of the whole plant by using standard phytochemical methods. Acute oral toxicity test was carried out and antiulcer activity was conducted using absolute ethanol and aspirin as the ulcerogenic agents on rats where the ulcer index was the parameter and percentage preventive index was determined. The aqueous ethanol extract of the plant was found to contain flavonoids,  anthraquinones, glycosidesand alkaloids. Acute toxicity test showed an oral LD₅₀ greater than 5000 mg/kg. In ethanol model, aqueous ethanol extract at doses of 250 and 500 mg/kg exhibited significant (P<0.0001) protection against ulcer with mean ulcer indices of 4.40 ± 0.60 and 1.80 ± 0.37 respectively as compared with negative (12.80 ± 0.97) and positive (4.00 ± 0.71)control mean ulcer indices. In aspirin model, aqueous ethanol extract at doses of 250 and 500 mg/kg demonstrated  significant (P<0.05) protection against ulcer with mean ulcer indices of 3.20 ± 0.80 and 2.60 ± 0.24 respectively ascompared with negative (5.60 ± 0.97) and positive (1.00 ± 0.44) controlmean ulcer indices. The higher dose of the extract demonstrated greater protective ability with percentage preventive index 85.94% in ethanol induced model and 53.57% in aspirin induced model. The aqueous ethanol extract of the plant was found to be non-toxic and contains some phytochemicals that could be responsible for its antiulcer activity.

Keywords: Thesiumviride, Phytochemical, LD₅₀, Ulcer index, Aqueous ethanol extract