Effect of oral administration of ethanolic extract of Tapinanthus globiferus A. rich on liver function in rats

Abstract

The effects of ethanol extract of Tapinanthus globiferus in the liver of rats were evaluated on serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total protein (TP), total bilirubin (TB) and conjugated bilirubin (CB) while histological analysis was evaluated on section of liver after 28 days of oral administration. The oral LD50 of the extract in rats was found to be greater than 5,000 mg/kg body weight. The biochemical analysis revealed no significant changes (p > 0.05) in serum levels of AST, ALT, ALP and TP at all doses treated (87.5, 175 and 350 mg/kg) but there was significant (p < 0.05) evaluation of serum TB at higher dose and significant (p < 0.05) dose dependent increase in CB when compared with control. The photomicrograph of transverse section of the liver treated with doses of 175 and 350 mg/kg showed areas of fibrosis at 100 magnifications while no effect was observed at 87.5 mg/kg body weight. This work revealed that, Tapinanthus globiferus extract has no toxic effect on the liver of rats after short and low dose administration but can cause sign of liver damage at higher dose.

Key words: Tapinanthus globiferus, Biochemical, Ethanol, Histological, Photomicrograph