Phytochemical and Antibacterial Studies of Cordia Africana Lam

Abstract

Cordia Africana antibacterial activity was determined using stem bark extract, which was tested against four bacterial isolates: Staphylococcus Aureus, Bacillus Subtilis, Escherichia Coli and Pseudomonas Aeruginosa using the agar well diffusion method. Ethyl acetate extract shows highest activity with 8.0 mm, zone of inhibition.TLC was carried out and the column chromatography profile yielded 97 fractions, which were pooled to six combined sub-fractions (A-F). The pure isolate A shows antibacterial activity on all the bacterial with highest zone of inhibition of 22.0 mm on Escherichia Coli. The spectroscopic studies revealed that isolate A consist of three compounds i.e. isobutyloctadecylester, butyl undecyl ester, methyl-12-oxo-9-dodecanoate