Evaluation of Interleukin 8 gene polymorphism for predicting inflammation in Indian chronic kidney disease and peritoneal dialysis patients

Abstract

Background and aim: Previous studies have observed the association between inflammation and chronic kidney disease (CKD). The role played by Interleukin 8 (IL8) gene polymorphism has not been studied yet. Hence, the present study has been designed as the first attempt to identify the possible associations between polymorphism of the IL-8 gene and patients with diabetic CKD and on continuous ambulatory peritoneal dialysis (CAPD).

Materials and methods: A total of 150 participants were selected from a private nephrology outpatient clinic. The subjects were divided into three groups: healthy individuals without any renal complications (group 1, control, n=50), patients with diabetic chronic kidney disease of stages 3 and 4 (group 2, n= 50) and CAPD (group 3, n= 50). Blood deoxyribo nucleic acid (DNA) isolated from the members of the study group, was confirmed by agarose gel electrophoresis and primers specific for IL8 gene were designed, using primer3 software tool.

Results: Restriction digestion of the amplicons with Escherichia coli restriction enzyme I (EcoRI) ended up in 203 base pairs (bp) band in control and 108 bp band in all diabetic and non-diabeticCKD. This indicated the presence of polymorphismin +781 Cytosine/Thymine (C/T) of IL-8 gene in diabetic CKD and CAPD patients. Statistical analysis of the distribution of frequencies of alleles C and T by chi square test confirmed the presence of polymorphismat+781C/T of IL-8 gene in patient groups compared to control.

Conclusion: The polymorphismin+781C/T of IL-8 gene studied in this work suggests its possible role as an inflammatory marker for both chronic kidney disease and CAPD.

Keywords: IL-8 gene polymorphism; CKD; CAPD; +781 C/T