Semen quality and histomorphological analysis of testicular tissues of rabbit bucks administered human menopausal gonadotropin

Abstract

The study investigated the effects of Menogon treatment on semen quality and histomorphology of testicular tissues in rabbit bucks. Twenty-four bucks of Chinchilla × Dutch breed, weighing 1.3 – 1.6 kg at 15 – 17 weeks of age, were divided into four groups receiving different Menogon doses (0.0 IU as control, 7.5 IU, 15.0 IU, and 22.5 IU) for 105 days in a completely randomised design (CRD). Each treatment group was replicated three times with two bucks per replicate. Semen quality and histological examination of the testes were assessed. Results showed that progressive sperm motility, sperm concentration, live sperm percentage,  and libido were significantly higher (p<0.05) in the group receiving 15.0 IU of Menogon compared to other groups. The percentage of total abnormal sperm was significantly lower in the 15.0 IU group compared to ther groups. Histological analysis revealed that the 15.0 IU treatment induced more hyperplasia of the germinal epithelium, which positively impacted semen production. These findings suggest that the optimal reproductive processes in rabbit bucks were observed at  a Menogon dose of 15.0 IU, indicating the potential of Menogon in enhancing testicular health and spermatogenic processes.