Karyotypic analysis of Labeo Coubie (Ruppell, 1832) (African Carp) from University of Ilorin Dam, Ilorin, Nigeria

Abstract

Karyotypic information is a useful endpoint in environmental monitoring and breeding programme. Data on karyotype of Labeo coubie is needful for environmental assessment and genetic improvement breeding projects. This study was aimed at determining the karyotype of L. coubie. 10 specimens were obtained from the University of Ilorin dam, Ilorin, Nigeria. Each specimen received intraperitoneally 0.02% colchicine (1ml/100g body weight) and left for 4 hours before sacrificing. Chromosome preparation was made from the kidney and liver. A total of 200 metaphase spreads were scored. The diploid chromosome numbers ranged from 2n = 44 to 2n = 50. The modal diploid number was found to be 2n = 50 and this represents 56 %. The kidney tissue gave better chromosome preparation. These results contribute to the karyotypic data on L. coubie.

Keywords: Labeo coubie, Karyotype, Chromosome