Preliminary cytological study of Amietophrynus regularis (Reuss, 1833) from Afon, Kwara State, Nigeria

Abstract

Information on the karyotype of Amietophrynus regularis is scarce. 8 specimens of A. regularis were collected from Afon, Kwara State, Nigeria. The specimens were injected intraperitoneally with 0.5 % colchicine and left for 5 hours before they were sacrificed. Chromosomes were prepared from the bone marrow of the femur. 80 metaphase spreads were scored and the diploid chromosome number ranged from 2n = 38 to 2n = 42. The modal number of chromosome was found to be 2n = 40 with percentage occurrence of 47.5 %.

Keywords: Amietophrynus regularis, Afon, Chromosome, Metaphase, Karyotype