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RP-HPLCdetermination of ginsenosides RG1 and RB1 in Panax ginseng fruits
Abstract
Background: At present, there has been a lot of research at home and abroad on the roots, stems and leaves of Panax Ginseng as well as their extracts, but the fruits of Panax Ginseng have been relatively little studied.
Materials and Methods: To establish a method for determination of ginsenosides Rg1 and Rb1 in Panax Ginseng fruits. RP-HPLC method is adopted, column used is a ZOBAX SB-C18 column (4.6 mm × 250 mnl, 5 μm), mobile phase A is water, and B is acetonitrile, gradient elution conditions are: 0~20 min (A:B 20:80), 20~60 min (A:B 20~35: 80~65); and detection wavelength 203 nm.
Results: Ginsenosides Rgl and Rb1 have good linear relationships within the ranges of 1.04~10.40 μg and 0.50~5.00 μg, respectively, and r is 0.9998 and 0.9997; reproducibility and recovery of the method are both in line with requirements.
Conclusion: The method established is simple, accurate and fast, which is suitable for the simultaneous determination of ginsenosides Rg1 and Rb1 in Panax Ginseng fruits.
Keywords: ginsenoside Rg1; ginsenoside Rb1; HLPC; leukemia
