Optical chlorophyll measurements as predictors of total nitrogen, nitrogen fractions and in vitro ruminal nitrogen degradability in tropical grass forages

Abstract

This study evaluated whether the FieldScout CM 1000 NDVI and Yara N–Tester models can produce accurate and reliable estimates of nitrogen (N), buffer-soluble nitrogen (BSN), buffer-insoluble nitrogen (BISN), non-protein nitrogen (NPN) and in vitro ruminal nitrogen degradability after 3, 12 and 24 h incubation (ND3, ND12 and ND24) in three tropical grasses: Brachiaria hybrid, Megathyrsus maximus and Paspalum atratum. Correlation between the Yara N-Tester and N, BISN and in vitro ruminal N degradability of the Brachiaria hybrid and M. maximus were high (r = 0.67–0.83). The Yara N-Tester accounted for 81% and 86% (p = 0.000) of N variability in the Brachiaria hybrid and M. maximus, respectively. The Yara N-Tester prediction models explained 72% and 70% (p = 0.000) BISN variability in the Brachiaria hybrid and M. maximus, respectively. In vitro ND24 of the Brachiaria hybrid (R² = 0.75) and M. maximus (R² = 0.75) was also best predicted with the Yara N-Tester. Model validation showed generally low (≤0.90) concordance correlation coefficients except for Yara N-Tester N and ND24 in M. maximus. Random error was the main source of error. We conclude that the accuracy of the Yara N-Tester prediction models was superior to that of the FieldScout CM 1000 NDVI models, and that the Yara N-Tester can produce accurate and reliable estimates of Brachiaria hybrid and M. maximus N and M. maximus ND24.

Keywords: buffer soluble/insoluble N, FieldScout CM 1000 NDVI, in vitro ruminal N degradability, nitrogen, non-protein nitrogen, Yara N-Tester