In vitro regeneration of Digitaria eriantha.

Abstract

Embryogenic callus was obtained from immature regions of the leaf of Digitaria eriantha when grown in the dark on a MS medium supplement with 1, 0 or 2, 0 mg/dm 3, 2, 4D and 3-9% sucrose. Plantlet regeneration occurred when the calli were transferred to MS medium containing 0, 1 mg/dm 3, 2, 4-D and 7-8% sucrose, and placed under 16 hour photoperiod. The regenerated plantlets were successfully planted out into a mixture of sand and potting soil.

Keywords: 2, 4-d; botany; callus; digitaria eriantha; embryogenic callus; leaves; photoperiod; plantlets; regeneration; sucrose